Adherence of Mycobacterium lepraemurium to Tissue Culture Cells

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タイトル ( eng )
Adherence of Mycobacterium lepraemurium to Tissue Culture Cells
タイトル ( jpn )
鼠癩菌の培養細胞への付着
作成者
Katoh Masafumi
Matsuo Yoshiyasu
収録物名
Hiroshima Journal of Medical Sciences
32
3
開始ページ 285
終了ページ 290
収録物識別子
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
抄録
Adherence of Mycobacterium lepraemurium to tissue culture cells was examined and compared with that of M. microti. M. lepraemurium used in the present study was maintained on 1% Ogawa egg yolk medium. This microbe adhered to HEp-2 cells much more than A31 or McCoy cells, though the adherence was unusually low as compared with the large infectious dose. Frequency distribution of the number of bacteria agreed nearly with Polya-Eggenberger distribution. The pretreatment of M lepraemurium with heat or protease increased the adherence of the bacteria to HEp-2 cells, whereas the pretreatment with lipase or hyaluronidase retained the adhering ability. The pretreatment of M. microti with heat or protease produced the same effect on the adherence as that of M. lepraemurium. These results suggest that adherence of M. lepraemurium to HEp-2 cells is prevented by protein-like material on the surface of the bacteria, and that the adherence is independent of specific adhesin-receptor interaction.
著者キーワード
Bacterial adherence
M. lepraemurium
M. microti
Tissue culture cells
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 紀要論文
出版者
Hiroshima University School of Medicine
発行日 1983-09
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6358140