Purification of Thiol Protease Inhibitor from Human Lung Cancer Tissue

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Title ( eng )
Purification of Thiol Protease Inhibitor from Human Lung Cancer Tissue
Title ( jpn )
ヒト肺癌組織からのチオールプロテアーゼインヒビターの精製
Creator
Okumichi Tsuneo
Nishiki Masayuki
Takasugi Sumiyoshi
Yamane Motoi
Ezaki Haruo
Source Title
Hiroshima Journal of Medical Sciences
Volume 33
Issue 4
Start Page 801
End Page 808
Journal Identifire
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
Abstract
It was confirmed that the thiol protease inhibitory activities in human lung cancer extracts were significantly higher than normal lung tissue extracts.

Furthermore the thiol protease inhibitor was extracted from human lung cancer tissue and purified by papain-Sepharose and Sephadex G-100 column chromatographies. The purified inhibitor inhibited papain and ficin, and its molecular weight determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was about 13,000.
Keywords
Thiol protease inhibitor
Human lung cancer
Descriptions
This research was funded in part by Grant-in-Aid for Scientific Research, Project 56480213, of the Japanese Ministry of Education.
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type departmental bulletin paper
Publisher
Hiroshima University School of Medicine
Date of Issued 1984-12
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6398816