A Method of In Vivo Simultaneous Measurements of Dopamine, Serotonin and Their Metabolites Using Intracerebral Microdialysis System

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タイトル ( eng )
A Method of In Vivo Simultaneous Measurements of Dopamine, Serotonin and Their Metabolites Using Intracerebral Microdialysis System
作成者
Yoshimoto Kanji
Komura Setsuo
Fujita Tomio
収録物名
Hiroshima Journal of Medical Sciences
37
1
開始ページ 41
終了ページ 43
収録物識別子
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
抄録
The present study demonstrated that it is possible to measure the endogenous extracellular dopamine (DA) and its metabolite dihydroxyphenylacetic acid (DOPAC), endogenous extracellular 5-hydroxytryptamine (5HT), its metabolites 5-hydroxyindoleacetic acid (5HIAA), 5-hydroxytryptophol (5HTOL) and its precursor 5-hydroxytryptophan (5HTP) simultaneously from the striatum of freely moving rats using intracerebral dialysis combined with high performance liquid chromatography (HPLC) with electrochemical detector (ECD).

Basal extracellular levels of DA, DOPAC, 5HTP, 5HT, 5HIAA and 5HTOL in the striatum were estimated to be 18.89 ±. 2.23 (pg/25 μl/60 min), 7.25 ± 0.32 (ng/25 μl/60 min), 13.35 ± 3.01 (pg/25 μl/60 min), 5.32 ± 0.92 (pg/25 μl/60 min), 2.41 ± 0.08 (ng/25 μl/60 min) and 7.69 ± 0.45 (pg/25 μl/60 min), respectively. Pargyline (75 mg/kg i.p.) and tryptophan (100 mg/kg i.p.) increased the 5HT level in the striatum perfusates by 3-fold, whereas DOPAC and 5HIAA levels decreased by approximately 20% and 8%, respectively. In addition, DA and 5HTOL levels were not changed.

This method is suitable for monitoring dopaminergic and serotonergic activities dynamically.
著者キーワード
Brain dialysis
Dopamine
Serotonin
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 紀要論文
出版者
Hiroshima University Medical Press
発行日 1988-03
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 3410734