Effect of OK-432 on the Lymphnode Metastasis of MCA-sarcoma Cell Lines: A New Therapeutic Approach

アクセス数 : 1103
ダウンロード数 : 134

今月のアクセス数 : 2
今月のダウンロード数 : 0
File
HiroshimaJMedSci_38_213.pdf 436 KB 種類 : fulltext
Title ( eng )
Effect of OK-432 on the Lymphnode Metastasis of MCA-sarcoma Cell Lines: A New Therapeutic Approach
Creator
Inoh Akira
Kodama Yuso
Yokoro Kenjiro
Source Title
Hiroshima Journal of Medical Sciences
Volume 38
Issue 4
Start Page 213
End Page 219
Journal Identifire
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
Abstract
The two unique highly metastatic MCA-sarcoma cell lines have been established by the present authors. The inoculation of 1153Ln, one of the cell lines, either into footpad or subcutaneously on the back of syngeneic mice resulted in the development of metastasis exclusively in almost all lymphnodes of the body. We evaluated the therapeutic effect of a streptococcal preparation, OK-432, on the lymphnode metastasis. Two KE per mouse of OK-432 were injected intratumorally (it) at 4, 7 or 10 days after the footpad inoculation of 1153Ln. OK-432 injected it at 7 days after tumor inoculation showed an inhibition of the lymphnode metastasis. Histological findings indicated that the proliferation of lymphoid cells in the drainage node was most prominent in mice treated with OK-432 at 7 days after the tumor inoculation. A combined treatment of it and intraperitoneal (ip) injections of OK-432 significantly reduced lymphnode metastasis as compared with that of ip injection alone. This can be attributed to the fact that the activated lymphocytes induced by it-injected OK-432 exhibited a potent antimetastatic activity together with general administration (ip) of OK-432 given after surgical removal of the tumor. Low dose of total-body irradiation (TBI), known to augment the antitumor potential of tumor-bearing animals together with general application of OK-432, showed synergistic action in inhibiting tumor growth. Overall results suggest that the better antitumor effect of OK-432 can be anticipated by combination of the agent itself and with other means.
Keywords
MCA-sarcoma
Lymphnode metastasis
OK-432
Low dose TBI
Descriptions
This work was supported by a Grant-in-Aid from the Ministry of Education, Science and Culture, Japan.
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type departmental bulletin paper
Publisher
Hiroshima University Medical Press
Date of Issued 1989-12
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 2637250