Pentane as an Index of in Vitro Lipid Peroxidation via Microsomal NADPH-P-450 Enzyme Systems
Hiroshima Journal of Medical Sciences Volume 38 Issue 3
Page 131-134
published_at 1989-09
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| File | |
| Title ( eng ) |
Pentane as an Index of in Vitro Lipid Peroxidation via Microsomal NADPH-P-450 Enzyme Systems
|
| Creator |
Sato Nobuyoshi
Fujii Kohyu
Yuge Osafumi
Morio Michio
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 38 |
| Issue | 3 |
| Start Page | 131 |
| End Page | 134 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
Pentane was measured in a mixture of guinea pig liver microsomes and carbontetrachloride (CC14) in the presence of nicotineamide adenine dinucleotide phosphate (NADPH) and under anaerobic conditions by gas chromatography. Pentane of 0.2 pmol/mg protein/min was formed in the presence of NADPH without CC14. Pentane formation increased to 2.0 pmol/mg protein/min in the presence of CC14 and NADPH. This reaction required an anaerobic atmosphere, 2.1 mmol/litter of NADPH and 13.4 mmol/litter of CC14 under optimal conditions. The formation was reduced in the presence of oxygen, glutathione, vitamin E and metyrapone. These results clearly show that pentane is formed by lipid peroxidation initiated by the free radical cleavage products of CC14, which are anaerobically produced by NADPH-dependent microsomal enzymes. We conclude that pentane is a good index of in vitro lipid peroxidation via microsomal NADPH-P-450 enzyme systems.
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| Keywords |
Lipid peroxides
Pentanes
Microsome
Liver
Carbontetrachloride
|
| Descriptions |
This study was supported in part by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University Medical Press
|
| Date of Issued | 1989-09 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 2584057
|
