Determination by Ion-exchange Chromatography of Trifluoroacetic Acid as a Biliary Metabolite of Isoflurane in the Rabbit
Hiroshima Journal of Medical Sciences Volume 38 Issue 1
Page 27-34
published_at 1989-03
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| File | |
| Title ( eng ) |
Determination by Ion-exchange Chromatography of Trifluoroacetic Acid as a Biliary Metabolite of Isoflurane in the Rabbit
|
| Creator |
Kawaguchi Ryoji
Fujii Kohyu
Morio Michio
Yuge Osafumi
Hossain Md. Delawar
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 38 |
| Issue | 1 |
| Start Page | 27 |
| End Page | 34 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
The optimal composition of eluant for trifluoroacetic acid (TFAA) analysis by ion-exchange chromatography was determined and a preliminary study to detect TFAA, a metabolite of isoflurane, in bile of rabbits during and after inhalation of isoflurane was made. The optimal composition of eluant for TFAA analysis by ion-exchange chromatography was determined to be a solution of 2rnM Na2C03 and 4mM NaHC03. Rabbits were divided into 4 groups; A group: 1 hr inhalation of 2% isoflurane, B group: 2 hr inhalation of 2% isoflurane, C group: 2 hr inhalation of 3% isoflurane, and D group: 2 hr inhalation of 4% isoflurane. The maximum biliary TFAA concentration in groups A, B, C and D averaged 17.5 ± 1.4 μM (mean ± S.D.) at 105 min, 37.1 ± 11.5 μM at 315 min, 54.5 ± 9.4 μM at 585 min and 34.4 ± 12.1 μM at 555 min after termination of inhalation. Cumulative excreted amounts of biliary TFAA for 20 hours were 746 nmols in A group (8 hours), 3,421 mnols in B group, 6,582 nmols in C group and 3,267 nmols in D group. Half-lives of biliary excreted TFAA were 63 min, 325 min, 478 min and 546 min, respectively. These results showed that ion-exchange chromatography is more convenient for detecting TFAA as a metabolite of isoflurane than isotachophoresis, gas chromatography, paper chromatography and thin layer chromatography and that TFAA was determined as a biliary metabolite of isoflurane in the rabbit.
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| Keywords |
Anesthetic
isoflurane
Chromatography
ion exchange
Biotransformation
trifluoroacetic acid
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University Medical Press
|
| Date of Issued | 1989-03 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 2753744
|
