Inhibition of 3H-TdR uptake for Human Gastric Carcinoma Cells by Epidermal Growth Factor Specific Antibody
Hiroshima Journal of Medical Sciences 40 巻 1 号
15-21 頁
1991-03 発行
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この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00038007
| ファイル情報(添付) | |
| タイトル ( eng ) |
Inhibition of 3H-TdR uptake for Human Gastric Carcinoma Cells by Epidermal Growth Factor Specific Antibody
|
| 作成者 |
Kyo Eikai
|
| 収録物名 |
Hiroshima Journal of Medical Sciences
|
| 巻 | 40 |
| 号 | 1 |
| 開始ページ | 15 |
| 終了ページ | 21 |
| 収録物識別子 |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| 抄録 |
This study examined whether human gastric carcinoma cells produced EGF for self-stimulation, using TMK-1 and MKN-28 cells which express mRNA for EGF and EGF receptor. EGF protein production was confirmed by ELISA and flow cytometric analysis. The lysates of these cells replaced EGF-induced biological activity to EGF receptor bearing KB cells but the culture supernatants did not. Furthermore, EGF-radioreceptor assay revealed non-secretion of EGF product. EGF specific monoclonal antibody neutralized exogenous EGF-induced 3H-TdR uptake, and inhibited spontaneous 3H-TdR uptake of TMK-1 and MKN-28 cells. These results suggest that EGF specific antibody binds to EGF protein at the cell surface, leading to the inhibition of 3HTdR uptake, and that EGF produced by tumor cells may act as an autocrine growth factor in human gastric carcinoma cells.
|
| 著者キーワード |
EGF
TGFα
Autocrine growth factor
Anti-EGF monoclonal antibody
|
| 内容記述 |
This study was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Health and Welfare for a Comprehensive 10-Year Strategy for Cancer Control, Japan.
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| NDC分類 |
医学 [ 490 ]
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| 言語 |
英語
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| 資源タイプ | 紀要論文 |
| 出版者 |
Hiroshima University Medical Press
|
| 発行日 | 1991-03 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 1864762
|
