Cytopathological and Immunohistochemical Study for Estimating Radiotherapeutic Effects in Uterine Cervical Cancer

アクセス数 : 716
ダウンロード数 : 77

今月のアクセス数 : 0
今月のダウンロード数 : 0
File
HiroshimaJMedSci_42_53.pdf 2.33 MB 種類 : fulltext
Title ( eng )
Cytopathological and Immunohistochemical Study for Estimating Radiotherapeutic Effects in Uterine Cervical Cancer
Creator
Begum Matia
Source Title
Hiroshima Journal of Medical Sciences
Volume 42
Issue 2
Start Page 53
End Page 65
Journal Identifire
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
Abstract
In order to estimate the effects of radiation on cancer tissue, cytopathological findings, BrdU labelling index (L.I.), tumor markers and C-myc oncogene products were examined in 30 patients of uterine cervical cancer, before radiation, at around 10Gy, 30Gy, 50Gy and at the end of the therapy. In order to assess the radiotherapeutic effects, the cytological, histological, immunohistological findings were evaluated by Papanicolaou's classification, Oboshi and Shimosato's grading system and BrdU L.I.  

In the cytology, the radiation effect, such as the enlargement and vacuolation of the nuclei and cytoplasm, was observed at around 10Gy of radiation, and significantly increased at around 30Gy showing lysis of the cells and pyknosis of the nuclei. The histological observation recorded remarkable change in the cancer tissue at around 30Gy of radiation. The BrdU L.I. showed a meaningful decrease around 30Gy in the radiosensitive cases compared with the radioresistant cases.  

Moreover, immunohistochemical investigation indicated the location of SCC and SLX in the cytoplasm of the cancer cells and of C-myc product in the nuclei. A distinct radiation effect was found in the C-myc oncogene product at around 10Gy. Ras oncogene was not found in the adenocarcinoma cases investigated.  

From these findings it is concluded that BrdU L.I., revealed by immunohistological study, is the most suitable indicator to estimate the response of radiation in cancer cells of the uterine cervix and that C-myc oncogene product has the potentiality to be used as a prognostic factor in uterine cervical cancer treated by radiation therapy.
Keywords
Radiotherapeutic effect
BrdU labelling index
Tumor antigen
Oncogene
Uterine cervical cancer
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type departmental bulletin paper
Publisher
Hiroshima University Medical Press
Date of Issued 1993-06
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0018-2052
[NCID] AA00664312