Restoration of IGFBP-rP1 Increases Radiosensitivity and Chemosensitivity in Hormone-refractory Human Prostate Cancer
Hiroshima Journal of Medical Sciences Volume 62 Issue 1
Page 13-19
published_at 2013-03
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Title ( eng ) |
Restoration of IGFBP-rP1 Increases Radiosensitivity and Chemosensitivity in Hormone-refractory Human Prostate Cancer
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Creator |
Seki Mitsuhiro
Mochizuki Hideki
Mutaguchi Kazuaki
Yasumoto Hiroaki
Oka Kiyotaka
Nagamatsu Hirotaka
Shoji Koichi
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Source Title |
Hiroshima Journal of Medical Sciences
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Volume | 62 |
Issue | 1 |
Start Page | 13 |
End Page | 19 |
Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
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Abstract |
We previously reported the tumor-suppressive activity of insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) through induction of apoptosis in human prostate cancer cells. The aim of this study was to investigate the effects of IGFBP-rP1 for radiosensitivity and chemosensitivity in hormone-refractory human prostate PC-3 cancer cells. Five assays were performed using PC-3 cells transfected with IGFBP-rP1 (PC-3rP1) and control cells transfected with an empty vector (PC-3N): PC-3rP1 and PC-3N were compared by clonogenic survival assay, cell cycle analysis and apoptotic assay for radiosensitivity. The number of colonies of PC-3rP1 cells significantly decreased after 4 and 8 Gy of irradiation, compared with those of PC-3N in the clonogenic survival assay. After 16 hr irradiation at 8 Gy, the percentage of apoptotic cells significantly increased in PC-3rP1 compared with PC-3N. Growth of PC-3rP1 was significantly lower than that of PC-3N after docetaxel treatment both in vitro and in vivo. These results indicate that restoration of IGFBP-rP1 to PC-3 cells increases both their radiosensitivity and chemosensitivity.
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Keywords |
IGFBP-rP1
Prostate cancer
Docetaxel
Radiosensitivity
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NDC |
Medical sciences [ 490 ]
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Language |
eng
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Resource Type | departmental bulletin paper |
Publisher |
Hiroshima University Medical Press
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Date of Issued | 2013-03 |
Rights |
(c) Hiroshima University Medical Press.
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Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
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