Restoration of IGFBP-rP1 Increases Radiosensitivity and Chemosensitivity in Hormone-refractory Human Prostate Cancer

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Title ( eng )
Restoration of IGFBP-rP1 Increases Radiosensitivity and Chemosensitivity in Hormone-refractory Human Prostate Cancer
Creator
Seki Mitsuhiro
Mochizuki Hideki
Mutaguchi Kazuaki
Yasumoto Hiroaki
Oka Kiyotaka
Nagamatsu Hirotaka
Shoji Koichi
Source Title
Hiroshima Journal of Medical Sciences
Volume 62
Issue 1
Start Page 13
End Page 19
Journal Identifire
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
Abstract
We previously reported the tumor-suppressive activity of insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) through induction of apoptosis in human prostate cancer cells. The aim of this study was to investigate the effects of IGFBP-rP1 for radiosensitivity and chemosensitivity in hormone-refractory human prostate PC-3 cancer cells. Five assays were performed using PC-3 cells transfected with IGFBP-rP1 (PC-3rP1) and control cells transfected with an empty vector (PC-3N): PC-3rP1 and PC-3N were compared by clonogenic survival assay, cell cycle analysis and apoptotic assay for radiosensitivity. The number of colonies of PC-3rP1 cells significantly decreased after 4 and 8 Gy of irradiation, compared with those of PC-3N in the clonogenic survival assay. After 16 hr irradiation at 8 Gy, the percentage of apoptotic cells significantly increased in PC-3rP1 compared with PC-3N. Growth of PC-3rP1 was significantly lower than that of PC-3N after docetaxel treatment both in vitro and in vivo. These results indicate that restoration of IGFBP-rP1 to PC-3 cells increases both their radiosensitivity and chemosensitivity.
Keywords
IGFBP-rP1
Prostate cancer
Docetaxel
Radiosensitivity
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type departmental bulletin paper
Publisher
Hiroshima University Medical Press
Date of Issued 2013-03
Rights
(c) Hiroshima University Medical Press.
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0018-2052
[NCID] AA00664312