Ontogeny of gene expression of group IB phospholipase A(2) isoforms in the red sea bream, Pagrus (Chrysophrys) major

Comparative Biochemistry and Physiology Part A : Molecular & Integrative Physiology 161 巻 2 号 185-192 頁 2011 発行
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タイトル ( eng )
Ontogeny of gene expression of group IB phospholipase A(2) isoforms in the red sea bream, Pagrus (Chrysophrys) major
作成者
Shimokawa Mariko
Satoh Fumi
Satoh Osamu
Yoshioka Daisuke
Aida Satoshi
Iijima Noriaki
収録物名
Comparative Biochemistry and Physiology Part A : Molecular & Integrative Physiology
161
2
開始ページ 185
終了ページ 192
抄録
The red sea bream (Pagrus major) was previously found to express mRNAs for two group IB phospholipase A(2) (PLA(2)) isoforms, DE-1 and DE-2, in the digestive organs, including the hepatopancreas, pyloric caeca, and intestine. To characterize the ontogeny of the digestive function of these PLA(2)s, the present study investigated the localization and expression of DE-1 and DE-2 PLA(2) genes in red sea bream larvae/juveniles and immature adults, by in situ hybridization. In the adults, DE-1 PLA(2) mRNA was expressed in pancreatic acinar cells. By contrast, DE-2 PLA(2) mRNA was detected not only in digestive tissues, such as pancreatic acinar cells, gastric glands of the stomach, epithelial cells of the pyloric caeca, and intestinal epithelial cells, but also in non-digestive ones, including cardiac and lateral muscle fibers and the cytoplasm of the oocytes. In the larvae, both DE-1 and DE-2 PLA(2) mRNAs first appeared in pancreatic tissues at 3 days post-hatching (dph) and in intestinal tissue at 1 dph, and expression levels for both gradually increased after this point. In the juvenile stage at 32 dph, DE-1 PLA(2) mRNA was highly expressed in pancreatic tissue, and DE-2 PLA(2) mRNA was detected in almost all digestive tissues, including pancreatic tissue, gastric glands, pyloric caeca, and intestine, including the myomere of the lateral muscles. In conclusion, both DE-1 and DE-2 PLA(2) mRNAs are already expressed in the digestive organs of red sea bream larvae before first feeding, and larvae will synthesize both DE-1 and DE-2 PLA(2) proteins.
著者キーワード
Adult
Group IB phospholipase A(2)
Isoform
In situ hybridization
Larva
Pagrus major
Teleost
NDC分類
生物科学・一般生物学 [ 460 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier Science INC
発行日 2011
権利情報
(c) 2011 Elsevier Inc. All rights reserved.
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 1095-6433
[DOI] 10.1016/j.cbpa.2011.10.022
[NCID] AA11183804
[DOI] http://dx.doi.org/10.1016/j.cbpa.2011.10.022