Upregulation of HOXA10 in gastric cancer with the intestinal mucin phenotype: reduction during tumor progression and favorable prognosis

Carcinogenesis Volume 33 Issue 5 Page 1081-1088 published_at 2012
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Title ( eng )
Upregulation of HOXA10 in gastric cancer with the intestinal mucin phenotype: reduction during tumor progression and favorable prognosis
Creator
Naito Yutaka
Sakamoto Naoya
Anami Katsuhiro
Oo Htoo Zarni
Uraoka Naohiro
Aoyagi Kazuhiko
Sasaki Hiroki
Source Title
Carcinogenesis
Volume 33
Issue 5
Start Page 1081
End Page 1088
Abstract
Gastric cancer (GC) is one of the most common malignancies worldwide. Better knowledge of the changes in gene expression that occur during gastric carcinogenesis may lead to improvements in diagnosis, treatment and prevention. In this study, we screened for genes upregulated in GC by comparing gene expression profiles from microarray and serial analysis of gene expression and identified the HOXA10 gene. The aim of the present study was to investigate the significance of HOXA10 in GC. Immunohistochemical analysis demonstrated that 221 (30%) of 749 GC cases were positive for HOXA10, whereas HOXA10 was scarcely expressed in non-neoplastic gastric mucosa except in the case of intestinal metaplasia. Next, we analyzed the relationship between HOXA10 expression and clinicopathological characteristics. HOXA10 expression showed a significant inverse correlation with the depth of invasion and was observed more frequently in the differentiated type of GC than in the undifferentiated type of GC. HOXA10 expression was associated with GC with the intestinal mucin phenotype and correlated with CDX2 expression. Furthermore, the prognosis of patients with positive HOXA10 expression was significantly better than in the negative expression cases. 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide and wound healing assay revealed that knockdown of HOXA10 in GC cells by short interfering RNA transfection significantly increased viability and motility relative to the negative control, indicating that HOXA10 expression inhibits cell growth and motility. These results suggest that expression of HOXA10 may be a key regulator for GC with the intestinal mucin phenotype.
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type journal article
Publisher
Oxford University Press
Date of Issued 2012
Rights
(c) The Author 2012. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com
Publish Type Author’s Original
Access Rights open access
Source Identifier
[ISSN] 0143-3334
[DOI] 10.1093/carcin/bgs121
[NCID] AA12072343
[DOI] http://dx.doi.org/10.1093/carcin/bgs121