Characterization of Acetonitrile-Tolerant Marine Bacterium Exiguobacterium sp SBH81 and Its Tolerance Mechanism

Microbes and Environments Volume 27 Issue 1 Page 30-35 published_at 2012
アクセス数 : 1008
ダウンロード数 : 245

今月のアクセス数 : 1
今月のダウンロード数 : 0
File
Microbes-and-Environments_27_30.pdf 689 KB 種類 : fulltext
Title ( eng )
Characterization of Acetonitrile-Tolerant Marine Bacterium Exiguobacterium sp SBH81 and Its Tolerance Mechanism
Creator
Ajiraporn Kongpol
Alisa S.Vangnai
Source Title
Microbes and Environments
Volume 27
Issue 1
Start Page 30
End Page 35
Abstract
A Gram-positive marine bacterium, Exiguobacterium sp. SBH81, was isolated as a hydrophilic organic-solvent tolerant bacterium, and exhibited high tolerance to various types of toxic hydrophilic organic solvents, including acetonitrile, at relatively high concentrations (up to 6% [v/v]) under the growing conditions. Investigation of its tolerance mechanisms illustrated that it does not rely on solvent inactivation processes or modification of cell surface characteristics, but rather, increase of the cell size lowers solvent partitioning into cells and the extrusion of solvents through the efflux system. A test using efflux pump inhibitors suggested that secondary transporters, i.e. resistance nodulation cell division (RND) and the multidrug and toxic compound extrusion (MATE) family, are involved in acetonitrile tolerance in this strain. In addition, its acetonitrile tolerance ability could be stably and significantly enhanced by repetitive growth in the presence of toxic acetonitrile. The marked acetonitrile tolerance of Exiguobacterium sp. SBH81 indicates its potential use as a host for biotechnological fermentation processes as well as bioremediation.
Keywords
organic-solvent tolerant bacteria
organic-solvent tolerant mechanisms
cell adaptation
Exiguobacterium
NDC
Biology [ 460 ]
Language
eng
Resource Type journal article
Publisher
Japanese Society of Microbial Ecology, Department of Bioresource Science
Date of Issued 2012
Rights
(c) 2012 the Japanese Society of Microbial Ecology (JSME) / the Japanese Society of Soil Microbiology (JSSM). All Rights are Reserved.
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 1342-6311
[DOI] 10.1264/jsme2.ME11228
[NCID] AA11551577
[DOI] http://dx.doi.org/10.1264/jsme2.ME11228