Characterization of Acetonitrile-Tolerant Marine Bacterium Exiguobacterium sp SBH81 and Its Tolerance Mechanism

Microbes and Environments 27 巻 1 号 30-35 頁 2012 発行
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ファイル情報(添付)
タイトル ( eng )
Characterization of Acetonitrile-Tolerant Marine Bacterium Exiguobacterium sp SBH81 and Its Tolerance Mechanism
作成者
Ajiraporn Kongpol
Alisa S.Vangnai
収録物名
Microbes and Environments
27
1
開始ページ 30
終了ページ 35
抄録
A Gram-positive marine bacterium, Exiguobacterium sp. SBH81, was isolated as a hydrophilic organic-solvent tolerant bacterium, and exhibited high tolerance to various types of toxic hydrophilic organic solvents, including acetonitrile, at relatively high concentrations (up to 6% [v/v]) under the growing conditions. Investigation of its tolerance mechanisms illustrated that it does not rely on solvent inactivation processes or modification of cell surface characteristics, but rather, increase of the cell size lowers solvent partitioning into cells and the extrusion of solvents through the efflux system. A test using efflux pump inhibitors suggested that secondary transporters, i.e. resistance nodulation cell division (RND) and the multidrug and toxic compound extrusion (MATE) family, are involved in acetonitrile tolerance in this strain. In addition, its acetonitrile tolerance ability could be stably and significantly enhanced by repetitive growth in the presence of toxic acetonitrile. The marked acetonitrile tolerance of Exiguobacterium sp. SBH81 indicates its potential use as a host for biotechnological fermentation processes as well as bioremediation.
著者キーワード
organic-solvent tolerant bacteria
organic-solvent tolerant mechanisms
cell adaptation
Exiguobacterium
NDC分類
生物科学・一般生物学 [ 460 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Japanese Society of Microbial Ecology, Department of Bioresource Science
発行日 2012
権利情報
(c) 2012 the Japanese Society of Microbial Ecology (JSME) / the Japanese Society of Soil Microbiology (JSSM). All Rights are Reserved.
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 1342-6311
[DOI] 10.1264/jsme2.ME11228
[NCID] AA11551577
[DOI] http://dx.doi.org/10.1264/jsme2.ME11228