Enrichment Using an Up-flow Column Reactor and Community Structure of Marine Anammox Bacteria from Coastal Sediment
Microbes and Environments Volume 26 Issue 1
Page 67-73
published_at 2011
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| File | |
| Title ( eng ) |
Enrichment Using an Up-flow Column Reactor and Community Structure of Marine Anammox Bacteria from Coastal Sediment
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| Creator |
Awata Takanori
Suzuki Yuji
Tanabe Katsuichiro
Hatamoto Masashi
Ohashi Akiyoshi
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| Source Title |
Microbes and Environments
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| Volume | 26 |
| Issue | 1 |
| Start Page | 67 |
| End Page | 73 |
| Abstract |
We established an enrichment culture of marine anaerobic ammonium oxidation (anammox) bacteria using an up-flow column reactor fed with artificial sea water supplemented with nitrogen and minerals and inoculated with coastal surface sediment collected from Hiroshima Bay. After 2 months of reactor operation, simultaneous removal of NH4+ and NO2- was observed, suggesting that an anammox reaction was proceeding. A total nitrogen removal rate of 2.17 g-N L-1 day-1 was attained on day 594 while the nitrogen loading rate was 3.33 g-N L-1 day-1. Phylogenetic analysis revealed that at least two dominant “Candidatus Scalindua" species were present in this reactor. Moreover, many uncultured bacteria and archaea, including candidate division or ammonia-oxidizing archaea, were present. Fluorescence in situ hybridization (FISH) revealed that anammox bacteria accounted for 85.5 ± 4.5% of the total bacteria at day 393. We also designed two oligonucleotide probes specific to each dominant “Candidatus Scalindua" species. A simultaneous FISH analysis using both probes showed that two different “Candidatus Scalindua" species were clearly recognizable and coexisted during reactor operation, although there was some variation in their abundance. The marine anammox bacteria enriched in this study have potential applications to the treatment of industrial wastewater containing high levels of ammonium and salt.
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| Keywords |
anaerobic ammonium oxidation (anammox)
marine anammox bacteria
“Candidatus Scalindua" species
enrichment culture
FISH
phylogenetic analysis
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| NDC |
Pollution. Environmental engineering [ 519 ]
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
日本微生物生態学会
日本土壌微生物学会
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| Date of Issued | 2011 |
| Rights |
Copyright (c) the Japanese Society of Microbial Ecology (JSME) / the Japanese Society of Soil Microbiology (JSSM)
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| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 1342-6311
[DOI] 10.1264/jsme2.ME10158
[NCID] AA11551577
[DOI] http://dx.doi.org/10.1264/jsme2.ME10158
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