Studies on genomic DNA flanking with trasgene in trasgenic Arabidopsis plants

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Title ( eng )
Studies on genomic DNA flanking with trasgene in trasgenic Arabidopsis plants
Title ( jpn )
形質転換シロイヌナズナ植物における外来遺伝子近傍のゲノムDNAの解析に関する研究
Creator
Sawasaki Tatsuya
Descriptions
CONTENTS
INTRODUCTION / p1
MATERIALS AND METHODS / p4
CHAPTER I STABLE TRANSFORMATION OF ARABIDOPSIS WITH THE BAR GENE USING PARTICLE BOMBARDMENT / p12
 Section1 Comparison of 3 selectable makers using changes in the number of drug-resistant calli on Arabidopsis root tissues / p12
 Section2 Regeneration of bialaphos-resistant Arabidopsis plants / p15
 Section3 Analyses of transgenic Arabidopsis plants / p17
 Section4 Analyses of morphology of transgenic Arabidopsis plants / p22
CHAPTER II CHARACTERIZATION OF JUNCTION REGIONS IN TRANSGENIC ARABIDOPSIS PLANTS OBTAINED BY PARTICLE BOMBARDMENT / p24
 Section1 Cloning of transgene and junction regions in transgenic Arabidopsis / p24
 Section2 Characterization of sequences of junction regions / p27
 Section3 In vitro binding assay of junction regions against tobacco matrices / p30
 Section4 Homology of junction regions / p35
 Section5 Characteristics of sequences of the CT junctions / p36
CHAPTER III INTEGRATION MODEL FOR THE TRANSGENE INTRODUCED BY PARTICLE BOMBARDMENT / p39
CONCLUSIONS / p42
ACKNOWLEDGMENTS / p44
REFERENCES / p45
NDC
Botany [ 470 ]
Language
eng
Resource Type doctoral thesis
Rights
Copyright(c) by Author
Publish Type Not Applicable (or Unknown)
Access Rights open access
Source Identifier
・Sawasaki, T., Seki, M., Anzai, H., Irifune, K. and Morikawa, H. (1994) Stable transformation of Arabidopsis with the bar gene using particle bombardment. Transgenic Res. 3, 279-286. references
・Sawasaki, T., Takahashi, M., Goshima, N. and Morikawa, H. Characterization of jucntion regions in transgenic Arabidopsis plants obtained by particle bombaredment. Gene (in press). references
[DOI] http://dx.doi.org/10.1007/s002990050432 references
[DOI] http://dx.doi.org/10.1016/S0378-1119(98)00388-6 references
Dissertation Number 甲第1905号
Degree Name
Date of Granted 1998-09-21
Degree Grantors
広島大学