Studies on genomic DNA flanking with trasgene in trasgenic Arabidopsis plants
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この文献の参照には次のURLをご利用ください : https://doi.org/10.11501/3164292
| File | |
| Title ( eng ) |
Studies on genomic DNA flanking with trasgene in trasgenic Arabidopsis plants
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| Title ( jpn ) |
形質転換シロイヌナズナ植物における外来遺伝子近傍のゲノムDNAの解析に関する研究
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| Creator |
Sawasaki Tatsuya
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| Descriptions |
CONTENTS
INTRODUCTION / p1 MATERIALS AND METHODS / p4 CHAPTER I STABLE TRANSFORMATION OF ARABIDOPSIS WITH THE BAR GENE USING PARTICLE BOMBARDMENT / p12 Section1 Comparison of 3 selectable makers using changes in the number of drug-resistant calli on Arabidopsis root tissues / p12 Section2 Regeneration of bialaphos-resistant Arabidopsis plants / p15 Section3 Analyses of transgenic Arabidopsis plants / p17 Section4 Analyses of morphology of transgenic Arabidopsis plants / p22 CHAPTER II CHARACTERIZATION OF JUNCTION REGIONS IN TRANSGENIC ARABIDOPSIS PLANTS OBTAINED BY PARTICLE BOMBARDMENT / p24 Section1 Cloning of transgene and junction regions in transgenic Arabidopsis / p24 Section2 Characterization of sequences of junction regions / p27 Section3 In vitro binding assay of junction regions against tobacco matrices / p30 Section4 Homology of junction regions / p35 Section5 Characteristics of sequences of the CT junctions / p36 CHAPTER III INTEGRATION MODEL FOR THE TRANSGENE INTRODUCED BY PARTICLE BOMBARDMENT / p39 CONCLUSIONS / p42 ACKNOWLEDGMENTS / p44 REFERENCES / p45 |
| NDC |
Botany [ 470 ]
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| Language |
eng
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| Resource Type | doctoral thesis |
| Rights |
Copyright(c) by Author
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| Publish Type | Not Applicable (or Unknown) |
| Access Rights | open access |
| Source Identifier |
・Sawasaki, T., Seki, M., Anzai, H., Irifune, K. and Morikawa, H. (1994) Stable transformation of Arabidopsis with the bar gene using particle bombardment. Transgenic Res. 3, 279-286.
references
・Sawasaki, T., Takahashi, M., Goshima, N. and Morikawa, H. Characterization of jucntion regions in transgenic Arabidopsis plants obtained by particle bombaredment. Gene (in press).
references
[DOI] http://dx.doi.org/10.1007/s002990050432
references
[DOI] http://dx.doi.org/10.1016/S0378-1119(98)00388-6
references
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| Dissertation Number | 甲第1905号 |
| Degree Name | |
| Date of Granted | 1998-09-21 |
| Degree Grantors |
広島大学
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