The casein peptide Asn-Pro-Trp-Asp-Gln enforces the intestinal tight junction partly by increasing occludin expression in Caco-2 cells

British Journal of Nutrition Volume 140 Issue 7 Page 951-956 published_at 2010-10
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Title ( eng )
The casein peptide Asn-Pro-Trp-Asp-Gln enforces the intestinal tight junction partly by increasing occludin expression in Caco-2 cells
Creator
Yasumatsu Hatsumi
Source Title
British Journal of Nutrition
Volume 140
Issue 7
Start Page 951
End Page 956
Abstract
We have demonstrated previously that Asn-Pro-Trp-Asp-Gln (NPWDQ, amino acids 107-111 of alpha s(2)-casein) inhibited allergen permeation, such as that demonstrated by ovalbumin, using Caco-2 cells as an in vitro human intestinal epithelial model and in mouse jejunal and ileal loops ex vivo. In the present study, the mechanism underlying this inhibitory activity was examined in Caco-2 cells. Transepithelial resistance value increased in response to the addition of increasing NPWDQ concentrations (10(-6)-10(-4) M), which suggests that this peptide enhanced epithelial barrier function. Next, changes in mRNA expression by the addition of NPWDQ (10(-6) M) were analysed in Caco-2 cells using the microarray method. NPWDQ up-regulated the expression of the occludin gene in cells, but the level of the genes of the claudin family and zonula occludens-1 (ZO-1) was unchanged. Increased protein expression of occludin, but not of claudin-1 or of ZO-1, was also observed. Therefore, it is suggested that NPWDQ up-regulated the expression of occludin in particular and enforced the tight junction barrier. These data imply that a food-derived peptide can fine-tune the epithelial barrier.
Keywords
Casein peptides
Tight junctions
Occludin
Caco-2
Microarrays
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type journal article
Publisher
Cambridge University Press
Date of Issued 2010-10
Rights
Copyright (c) The Authors 2010
Publish Type Author’s Original
Access Rights open access
Source Identifier
[ISSN] 0007-1145
[DOI] 10.1017/S0007114510001698
[NCID] AA00574708
[DOI] http://dx.doi.org/10.1017/S0007114510001698