CZEエレクトロフェログラムの標準化 <講演 : キャピラリー電気泳動と生命解析科学>

Standardization of electropherograms of CZE

Hirokawa Takeshi

Ikuta Natsuki

Yoshiyamam Tatsuya

Okamoto Hikaru

Shibayama Takayuki

Manabe Yuuki

生物物理化学

Although chromatography is widely utilized, it is behind the other analytical methods from the viewpoint of the standardization of the data. This is because usual chromatograms are strongly dependent on the used hardware. Capillary electrophoresis is not either the exception: The obtained electropherograms are also dependent on the hardware such as capillary length, capillary inner surface, applied voltage, and thermostatting capacity etc, even if the same background electrolyte and the same sample is used. We have developed a conversion method of the time-based electropherograms of capillary zone electrophoresis (CZE) into the mobility-based ones by removing contribution of electroosmotic flow considering temperature rise caused by Joule heating. The conversion method also contained correction for delay of migration time caused by relaxation of potential gradient at sample plug. In this paper, after discussing the factors affecting migration time of CZE, electropherograms of rare-earth ions obtained using different migration voltage were converted to demonstrate utility of our method proposed for standardization (data transfer). The conversion method was also successfully applied to electropherograms of several rations obtained by using field enhanced sample stacking.

CZE (capillary zone electrophoresis)

electropherograms

conversion

standardization

stacking

Chemical technology [ 570 ]

jpn

日本電気泳動学会

Copyright (c) 2001 日本電気泳動学会

利用は著作権の範囲内に限られます

本文データはJournal@rchiveから複製したものである

[ISSN] 0031-9082

[DOI] 10.2198/sbk.45.103

[NCID] AN00129729

[DOI] http://dx.doi.org/10.2198/sbk.45.103