Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes
Clinical Biochemistry 43 巻 3 号
302-306 頁
2010-02 発行
アクセス数 : 728 件
ダウンロード数 : 245 件
今月のアクセス数 : 2 件
今月のダウンロード数 : 3 件
この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00029228
| ファイル情報(添付) | |
| タイトル ( eng ) |
Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes
|
| 作成者 |
Kinoshita-Kikuta Emiko
Nakashima Hiromi
|
| 収録物名 |
Clinical Biochemistry
|
| 巻 | 43 |
| 号 | 3 |
| 開始ページ | 302 |
| 終了ページ | 306 |
| 抄録 |
Objective: It is clinically important to detect the single-nucleotide polymorphism (SNP) of CYP3A5*3 (6986A>G) associated with enzymatic activity for drug metabolism. The aim of this study was to establish an accurate strategy for SNP screening. Design and methods: Polyacrylamide gel electrophoresis (PAGE) using zinc(II) complexes were applied for SNP detection. Genomic analyses of 19 healthy subjects were conducted by using both Zn2+-Phos-tag-PAGE and Zn2+-cyclen-PAGE methodologies. Results: Zn2+-Phos-tag PAGE permitted identification of the following allele genotypes: the A/A homozygote (CMA5*1/*1) in 3 individuals, the G/G homozygote (CMA5*3/*3) in 14 individuals, and the A/G heterozygote (CMA5*1/*3) in 2 individuals. Zn2+-cyclen PAGE demonstrated not only reproducibility of the genotyping but also existence of a novel heterozygous SNP (6929G>A) in the subject with CMA5*1/*1. Conclusion: We demonstrated reliable SNP genotyping and mapping in CYP3A5 using the combination method of Zn2+-Phos-tag PAGE and Zn2+-cyclen PAGE.
|
| 著者キーワード |
CYP3A5
SNP
Mutation
Zinc(II) complex
Phos-tag
Cyclen
PAGE
Electrophoresis
|
| NDC分類 |
生物科学・一般生物学 [ 460 ]
|
| 言語 |
英語
|
| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Pergamon
Elsevier Science Ltd
|
| 発行日 | 2010-02 |
| 権利情報 |
Copyright (c) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
|
| 出版タイプ | Author’s Original（十分な品質であるとして、著者から正式な査読に提出される版） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0009-9120
[DOI] 10.1016/j.clinbiochem.2009.09.007
[NCID] AA00607603
[DOI] http://dx.doi.org/10.1016/j.clinbiochem.2009.09.007
|
