DNA microarray analyses of genes expressed differentially in 3T3-L1 adipocytes co-cultured with murine macrophage cell line RAW 264.7 in the presence of the toll-like receptor 4 ligand bacterial endotoxin

International Journal of Obesity 32 巻 11 号 1725-1729 頁 2008 発行
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タイトル ( eng )
DNA microarray analyses of genes expressed differentially in 3T3-L1 adipocytes co-cultured with murine macrophage cell line RAW 264.7 in the presence of the toll-like receptor 4 ligand bacterial endotoxin
作成者
Yamashita Akiko
Soga Yoshihiko
Iwamoto Yoshihiro
Asano T.
Li Y.
Abiko Yoshimitsu
収録物名
International Journal of Obesity
32
11
開始ページ 1725
終了ページ 1729
抄録
Recent studies have suggested macrophages were integrated to adipose tissues to interact with adipocytes, thereby exacerbating inflammatory responses. Furthermore, both adipocytes and macrophages appear to express TLR-4, and free fatty acids may stimulate cells via TLR-4. Herein, we analyzed genes differentially expressed in adipocytes when co-cultured with macrophages in the presence of a ligand for TLR-4, bacterial lipopolysaccharide. RAW264.7, a murine macrophage cell line and differentiated 3T3-L1 adipocytes were co-cultured using a transwell system. Genes differentially expressed in adipocytes were analyzed by the DNA microarray method following 4, 8, 12 and 24h stimulation with 1 ng/ml of E. coli LPS. Randomly selected genes with high expressions were confirmed by quantitative methods at both the gene and the protein level. Co-culture of macrophages and adipocytes with a low LPS concentration (1 ng/ml) markedly up-regulated gene expressions associated with inflammation and/or angiogenesis, such as those of IL-6, MCP-1, RANTES and CXCL1/KC, in adipocytes. Furthermore, several genes associated with insulin resistance were differentially expressed. Up-regulations of genes encoding MCP-1, RANTES and CXC/KC were confirmed by quantitative methods. These results suggest that ligands for TLR-4 stimulate both adipocytes and macrophages to up-regulate the expressions of many genes associated with inflammation and/or angiogenesis.
著者キーワード
adipocyte-macrophage interaction
TLR-4; endotoxin
gene expression
microarray analyses
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Nature Publishing Group
発行日 2008
権利情報
Copyright (c) 2009 Nature Publishing Group
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0307-0565
[DOI] 10.1038/ijo.2008.153
[NCID] AA00680691
[DOI] http://dx.doi.org/10.1038/ijo.2008.153