Neutralizing Monoclonal Antibodies to Striped Jack Nervous Necrosis Virus (SJNNV)
魚病研究 30 巻 2 号
111-114 頁
1995-06 発行
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この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00025888
| ファイル情報(添付) | |
| タイトル ( eng ) |
Neutralizing Monoclonal Antibodies to Striped Jack Nervous Necrosis Virus (SJNNV)
|
| 作成者 |
Nishizawa Toyohiko
Kise Masaaki
Muroga Kiyokuni
|
| 収録物名 |
魚病研究
Fish Pathology
|
| 巻 | 30 |
| 号 | 2 |
| 開始ページ | 111 |
| 終了ページ | 114 |
| 抄録 |
Viral nervous necrosis (VNN) caused by a nodavirus (striped jack nervous necrosis virus: SJNNV) is a serious problem in seed production process of striped jack Pseudocaranx dentex in Japan. Mouse monoclonal antibodies (MAbs) to SJNNV were produced by using a homogenate of infected larval striped jack. Among 8 MAbs reacting with the homogenate of infected larvae in enzyme-linked immunosorbent assay (ELISA), three MAbs (SJ-102B, SJ-204D, SJ-207C) were proved to recognize the 42 kDa coat protein of SJNNV by Western blot analysis. An in vivo neutralization test, where larval striped jack were exposed to purified SJNNV (100 ng/ml) or the virus treated with MAbs (1 mg) and the resultant infection with SJNNV in larvae was monitored by ELISA using an anti-SJNNV rabbit serum, exhibited that two MAbs SJ-102B and SJ-204D have neutralizing activity against SJNNV.
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| 著者キーワード |
monoclonal antibody
nodavirus
SJNNV
neutralization
striped jack
viral nervous necrosis
VNN
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| NDC分類 |
水産業 [ 660 ]
|
| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
日本魚病学会
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| 発行日 | 1995-06 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0388-788X
[NCID] AN00063165
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