Serological relationships among genotypic variants of betanodavirus.

Diseases of Aquatic Organisms Volume 57 Issue 1-2 Page 19-26 published_at 2003-12-03
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Title ( eng )
Serological relationships among genotypic variants of betanodavirus.
Creator
Mori Koh-ichiro
Mangyoku Tomio
Iwamoto Tokinori
Arimoto Misao
Source Title
Diseases of Aquatic Organisms
Volume 57
Issue 1-2
Start Page 19
End Page 26
Abstract
Betanodaviruses, the causative agents of viral nervous necrosis or viral encephalopathy and retinopathy, are divided into 4 genotypes based on the coat protein gene (RNA2). In the present study, serological relationships among betanodavirus genotypic variants were examined by virus neutralization tests using rabbit antisera raised against purified virions of strains representative of each genotype. All 20 isolates examined shared epitopes for neutralizing, but they fell into 3 major serotypes (A, B, C). This sero-grouping is in part consistent with their genotypes, i.e. Serotype A for striped jack nervous necrosis virus (SJNNV) genotype, Serotype B for tiger puffer nervous necrosis virus (TPNNV) genotype, and Serotype C for both redspotted grouper nervous necrosis virus (RGNNV) and barfin flounder nervous necrosis virus (BFNNV) genotypes. The serological relatedness between RGNNV and BFNNV genotypes may result from their relatively higher similarity in RNA2 sequences. In neutralization tests using antisera of kelp grouper Epinephelus moara, which were raised against recombinant coat proteins representing each genotype, anti-SJNNV and anti-TPNNV sera neutralized only the homologous strain, and anti-RGNNV and anti-BFNNV sera reacted with both RGNNV and BFNNV strains. The present serological findings will be important in investigating the infectivity and host-specificity of betanodaviruses and in developing vaccines for the disease.
Keywords
Betanodavirus
Viral nervous necrosis
Viral encephalopathy
Viral retinopathy
Serotyping
Virus neutralization
Recombinant protein
NDC
Zoology [ 480 ]
Language
eng
Resource Type journal article
Publisher
Inter-Research
Date of Issued 2003-12-03
Rights
Copyright (c) 2003 Inter-Research.
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0177-5103
[ISSN] 1616-1580
[DOI] 10.3354/dao057019
[NCID] AA10443976
[DOI] http://dx.doi.org/10.3354/dao057019