Cloning of full-length cDNA of teleost corticotropin-releasing hormone precursor by improved inverse PCR
Bioscience, Biotechnology, and Biochemistry Volume 70 Issue 8
Page 1983-1986
published_at 2006
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| File | |
| Title ( eng ) |
Cloning of full-length cDNA of teleost corticotropin-releasing hormone precursor by improved inverse PCR
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| Creator |
Fukui Yukio
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| Source Title |
Bioscience, Biotechnology, and Biochemistry
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| Volume | 70 |
| Issue | 8 |
| Start Page | 1983 |
| End Page | 1986 |
| Abstract |
We designed a new inverse PCR protocol combined with switching mechanism at 5′ end of RNA transcript (SMART) technology, and applied it to the cloning of teleost corticotropin-releasing hormone precursor cDNA. Due to the advantages of both techniques, this method can efficiently amplify the complete 5′- and 3′-ends of cDNA in a single reaction, and might prove to be an alternative to the conventional rapid amplification of cDNA ends (RACE) approaches.
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| Keywords |
inverse PCR
corticotropin-releasing hormone precursor
teleost
Halichoeres tenuispinnis
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| NDC |
Fishing industry. Fisheries [ 660 ]
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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| Date of Issued | 2006 |
| Rights |
Copyright (c) 2006 Japan Society for Bioscience, Biotechnology, and Agrochemistry
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| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[DOI] 10.1271/bbb.60067
[NCID] AA10824164
[ISSN] 0916-8451
[DOI] http://dx.doi.org/10.1271/bbb.60067
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