Expression and activity of Runx2 mediated by hyaluronan during chondrocyte differentiation

Archives of Oral Biology 53 巻 5 号 478-487 頁 2008-05 発行
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タイトル ( eng )
Expression and activity of Runx2 mediated by hyaluronan during chondrocyte differentiation
作成者
Tanaka Nobuaki
Ueki Masashi
Lin Y. Y.
Ohkuma S
Kamiya Takashi
Tanaka Eiji
Tanne Kazuo
収録物名
Archives of Oral Biology
53
5
開始ページ 478
終了ページ 487
抄録
During endochondral ossification, the production of hyaluronan (HA) is strictly and selectively regulated by chondrocytes, with a temporal peak at the hypertrophic stage. This study was conducted to clarify the effects of HA on expression and activity of runt-related gene 2 (Runx2), a potent transcription factor for chondrocyte differentiation in hypertrophic chondrocytes. Immature chondrocytes from an ATDC5 cell line were cultured and differentiated in DMEM/Ham's F12 with pre-defined supplements. Using real-time PCR, the gene expressions of type II collagen, MMP-13, HAS2, and Runx2 in cultured chondrocytes were analysed from days 0 to 18 of cell differentiation. The activity and expression of Runx2 in hypertrophic chondrocytes were analysed after the treatment with HA oligosaccharide (HAoligo) using AML-3/Runx2 binding, real-time PCR and Western blot analysis. The effects of pre-incubation of anti-CD44 antibody on Runx2 expression were also examined. Expression of type X collagen and Runx2 mRNAs reached a maximum at the terminal differentiation of chondrocytes. The activity and expression of Runx2 was significantly inhibited in hypertrophic chondrocytes treated with HAoligo compared to the untreated controls. High molecular weight-HA did not affect the expression or activity of Runx2. The expression of Runx2 mRNA was significantly decreased in hypertrophic chondrocytes treated with anti-CD44 antibody. These results suggest that HAoligo may affect the terminal differentiation of chondrocytes during the endochondral ossification by inhibiting the expression and activity of Runx2.
著者キーワード
Runt-related gene 2 (Runx2)
Hyaluronan (HA)
Chondrocyte
Differentiation
HAoligo
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier Ltd
発行日 2008-05
権利情報
Copyright (c) 2007 Elsevier Ltd
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0003-9969
[DOI] 10.1016/j.archoralbio.2007.12.007
[NCID] AA00548457
[PMID] 18242579
[DOI] http://dx.doi.org/10.1016/j.archoralbio.2007.12.007 ~の異版である