Retention of Multilineage Differentiation Potential of Mesenchymal Cells during Proliferation in Response to FGF

Biochemical and Biophysical Research Communications 288 巻 2 号 413-419 頁 2001-10-26 発行
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タイトル ( eng )
Retention of Multilineage Differentiation Potential of Mesenchymal Cells during Proliferation in Response to FGF
作成者
Tsutsumi Shinichi
Miyazaki Kazuko
Pan Haiou
Koike Chika
Yoshida Eri
Takagishi Kenji
Kato Yukio
収録物名
Biochemical and Biophysical Research Communications
288
2
開始ページ 413
終了ページ 419
抄録
Mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells may be useful for autologous cell transplantation to defects of bone, cartilage, and tendon, if MSC can be expanded in vitro. However, a short life span of MSC and a reduction in their differentiation potential in culture have limited their clinical application. The purpose of this study is to identify a growth factor(s) involved in self-renewal of MSC and the maintenance of their multilineage differentiation potential. Fibroblast growth factor-2 (FGF-2) markedly increased the growth rate and the life span of rabbit, canine, and human bone marrow MSC in monolayer cultures. This effect of FGF-2 was more prominent in low-density cultures than in high-density cultures. In addition, all MSC expanded in vitro with FGF-2, but not without FGF-2, differentiated to chondrocytes in pellet cultures. The FGF(+) MSC also retained the osteogenic and adipogenic potential throughout many mitotic divisions. These findings suggest that FGFs play a crucial role in self-renewal of MSC.
著者キーワード
mesenchymal stem cell
FGF
multilineage differentiation potential
life span
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier
発行日 2001-10-26
権利情報
Copyright (c) 2001 Academic Press
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0006-291X
[DOI] 10.1006/bbrc.2001.5777
[NCID] AA00564395
[DOI] http://dx.doi.org/10.1006/bbrc.2001.5777