Characterization of CO3Ap-collagen sponges using X-ray high-resolution microtomography
Biomaterials 25 巻 13 号
2577-2583 頁
2004-06 発行
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Biomaterials_25_2577.pdf
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タイトル ( eng ) |
Characterization of CO3Ap-collagen sponges using X-ray high-resolution microtomography
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作成者 |
Itoh Manabu
Yoshida Yasuhiro
Shintani Hideaki
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収録物名 |
Biomaterials
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巻 | 25 |
号 | 13 |
開始ページ | 2577 |
終了ページ | 2583 |
抄録 |
For reconstruction and regeneration of hard tissues, scaffold biomaterials with large size pores and high porosity are important, in addition to their roles as supporting frames. To develop a new biodegradable scaffold biomaterial, CO3Ap, which has crystallinity and a chemical composition similar to bone, was synthesized at pH 7.4 and 60°C. Then, the CO3Ap was mixed with a neutralized collagen gel and the CO3Ap-collagen mixtures with different kinds of CO3Ap contents and porosity were lyophilized into sponges. Scanning electron micrography (SEM) observation of CO3Ap-collagen sponges showed favorable pores for cell invasion. Approximately 50–300 μm size pores appeared to continue through the bulk. Higher magnification of the sponge showed a better adhesion between CO3Ap crystals and collagen. X-ray high-resolution microtomography revealed a clear image of the 3D structure of the sponges. The porosity of 0, 70 and 90%(w/w) CO3Ap-collagen sponges was 79.2±2.8%, 72.6±2.4% and 48.9±6.1%, respectively. The 70%(w/w) CO3Ap-collagen sponge appeared to be the most favorable biomaterial from the viewpoint of natural bone properties. Mouse osteoblast MC3T3-E1 cells were cultured in αMEM with 10% FCS for 2 weeks. Hematoxylin–eosin staining confirmed osteoblast cells invaded well into the CO3Ap-collagen sponge. These sponges are expected to be used as hard tissue scaffold biomaterials for therapeutic uses.
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著者キーワード |
Bone repair
Collagen
Hydroxyapatite composite
Scaffold
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NDC分類 |
医学 [ 490 ]
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言語 |
英語
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資源タイプ | 学術雑誌論文 |
出版者 |
Elsevier
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発行日 | 2004-06 |
権利情報 |
Copyright (c) 2003 Elsevier Ltd.
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出版タイプ | Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版) |
アクセス権 | オープンアクセス |
収録物識別子 |
[ISSN] 0142-9612
[DOI] 10.1016/j.biomaterials.2003.09.071
[NCID] AA00110092
[DOI] http://dx.doi.org/10.1016/j.biomaterials.2003.09.071
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