One of the duplicated matrix metalloproteinase-9 genes is expressed in regressing tail during anuran metamorphosis

Development Growth and Differentiation 48 巻 4 号 223-241 頁 2006-05 発行
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タイトル ( eng )
One of the duplicated matrix metalloproteinase-9 genes is expressed in regressing tail during anuran metamorphosis
作成者
Fujimoto Kenta
収録物名
Development Growth and Differentiation
48
4
開始ページ 223
終了ページ 241
抄録
The drastic morphological changes of the tadpole are induced during the climax of anuran metamorphosis, when the concentration of endogenous thyroid hormone is maximal. The tadpole tail, which is twice as long as the body, shortens rapidly and disappears completely in several days. We isolated a cDNA clone, designated as Xl MMP-9TH, similar to the previously reported Xenopus laevis MMP-9 gene, and showed that their Xenopus tropicalis counterparts are located tandemly about 9 kb apart from each other in the genome. The Xenopus MMP-9TH gene was expressed in the regressing tail and gills and the remodeling intestine and central nervous system, and induced in thyroid hormone-treated tail-derived myoblastic cultured cells, while MMP-9 mRNA was detected in embryos. Three thyroid hormone response elements in the distal promoter and the first intron were involved in the upregulation of the Xl MMP-9TH gene by thyroid hormone in transient expression assays, and their relative positions are conserved between X. laevis and X. tropicalis promoters. These data strongly suggest that the MMP-9 gene was duplicated, and differentiated into two genes, one of which was specialized in a common ancestor of X. laevis and X. tropicalis to be expressed in degenerating and remodeling organs as a response to thyroid hormone during metamorphosis.
著者キーワード
Gene duplication
Matrix metalloproteinase-9
Metamorphosis
Programmed cell death
Thyroid hormone
NDC分類
動物学 [ 480 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Blackwell
発行日 2006-05
権利情報
Copyright (c) 2006 Authors, The definitive version is available at www.blackwell-synergy.com
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0012-1592
[DOI] 10.1111/j.1440-169X.2006.00859.x
[NCID] AA00627804
[DOI] http://dx.doi.org/10.1111/j.1440-169X.2006.00859.x ~の異版である