Simultaneous monitoring of inorganic cations, amines and amino acids in human sweat by capillary electrophoresis

Analytica Chimica Acta 581 巻 1 号 83-88 頁 2007-01-02 発行
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タイトル ( eng )
Simultaneous monitoring of inorganic cations, amines and amino acids in human sweat by capillary electrophoresis
作成者
Okamoto Hikaru
Gosyo Yoshinori
Tsuda Takao
Timerbaev Andrei R.
収録物名
Analytica Chimica Acta
581
1
開始ページ 83
終了ページ 88
抄録
The determination of cationic constituents of sweat is widely recognized as a difficult analytical task due to its complex composition and minute sample volumes available for the individual analysis. Capillary electrophoresis (CE) has been evaluated as a simple routine method to measure sweat metal cations, biogenic amines, and amino acids using a sampling procedure previously developed in one of collaborative teams. The carrier electrolyte, which consisted of 10 mM 4-methylbenzylamine, 6.5 mM α-hydroxyisobutyric acid, and 2 mM 18-crown-6 at pH 4.25 allowed the separation of five cations (NH4+, K+, Ca2+, Na+, Mg2+) and four amino acids (ornithine, histidine, lysine, arginine) to be completed in about 13 min with a positive polarity of the applied voltage (30 kV). By increasing the sample volume (due to employing hydrodynamic instead of hydrostatic injection mode), it was also possible to detect indirect UV signals of Zn2+, diethanolamine, and trietanolamine. Sweat samples were collected from the fingers and forearms of three healthy male volunteers and analyzed by CE. A good repeatability and reproducibility of peak area responses based on five intraday and three inter-day assays (average %RSD less than 3.5 and 2.5, respectively) were obtained. The limits of detection were in the range of 3.2-5.8 μM for alkali and alkaline-earth cations (hydrostatic injection) and 0.27-0.79 μM for other target analytes (hydrodynamic injection). The analytical results for particular analytes were found to vary, depending on the sampling spot and individual, but in general correspond well to clinical concentration ranges.
著者キーワード
Human sweat
Capillary electrophoresis
Sampling
Cations
Amino acids
Amines
NDC分類
化学 [ 430 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier Science B.V.
発行日 2007-01-02
権利情報
Copyright (c) 2006 Elsevier B.V.
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0003-2670
[DOI] 10.1016/j.aca.2006.07.077
[NCID] AA00524834
[DOI] http://dx.doi.org/10.1016/j.aca.2006.07.077