Abundance of the RSC nucleosome-remodeling complex is important for the cells to tolerate DNA damage in Saccharomyces cerevisiae
FEBS Letters 531 巻 2 号
215-221 頁
2002-11-06 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Abundance of the RSC nucleosome-remodeling complex is important for the cells to tolerate DNA damage in Saccharomyces cerevisiae
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| 作成者 |
Koyama Hirofumi
Itoh Masayuki
Miyahara Kohji
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| 収録物名 |
FEBS Letters
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| 巻 | 531 |
| 号 | 2 |
| 開始ページ | 215 |
| 終了ページ | 221 |
| 抄録 |
The essential Nps1p/Sth1p is a catalytic subunit of the nucleosome-remodeling complex, RSC, of Saccharomyces cerevisiae that can alter nucleosome structure by using the energy of ATP hydrolysis. Besides the ATPase domain, Nps1p harbors the bromodomain, of which the function(s) have not yet been defined. We have isolated a temperature-sensitive mutant allele of NPS1, nps1-13, which has amino acid substitutions within the bromodomain. This mutation perturbed the interaction between the RSC components and enhanced the sensitivity of the cells to several DNA-damaging treatments at the permissive temperature. Reduced expression of NPS1 also caused DNA damage sensitivity. These results suggest the importance of the Nps1p bromodomain in RSC integrity and a model in which high amounts of RSC would be required for the cells to overcome DNA damage
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| 著者キーワード |
RSC
nucleosome-remodeling complex
NPS1/STH1
Saccharomyces cerevisiae
bromodomain
DNA damage
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| NDC分類 |
生物科学・一般生物学 [ 460 ]
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Elsevier
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| 発行日 | 2002-11-06 |
| 権利情報 |
Copyright (c) 2002 Federation of European Biochemical Societies
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| 出版タイプ | Author’s Original（十分な品質であるとして、著者から正式な査読に提出される版） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[NCID] AA00642943
[ISSN] 0014-5793
[DOI] 10.1016/S0014-5793(02)03504-4
[DOI] http://dx.doi.org/10.1016/S0014-5793(02)03504-4
～の異版である
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