Identification of prostatic secreted proteins in mice by mass spectrometric analysis and evaluation of lobe-specific and androgen-dependent mRNA expression

Journal of Endocrinology 190 巻 3 号 793-803 頁 2006-09 発行
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タイトル ( eng )
Identification of prostatic secreted proteins in mice by mass spectrometric analysis and evaluation of lobe-specific and androgen-dependent mRNA expression
作成者
Akimoto Yukimi
Suzuki Tomoharu
Kitamura Shigeyuki
収録物名
Journal of Endocrinology
190
3
開始ページ 793
終了ページ 803
抄録
Rats and guinea pigs have frequently been used to study the development of the prostate and the mechanism of androgen action, but the mouse prostate has also become an attractive model for prostate research, because an enormous range of genetically altered mice is now available. However, the secretion of proteins in the mouse prostate has not yet been well investigated. In the present study, major secreted proteins from the ventral (VP), dorso-lateral (DLP) and anterior prostate (AP) of mice were identified by means of two-dimensional gel electrophoresis followed by MALDI-TOF mass spectrometric analysis. A quantitative RT-PCR method was further employed to examine the androgen-dependent transcriptional regulation of the identified proteins. Proteome analysis revealed that the VP secretes spermine binding protein, serine protease inhibitor Kazal type 3 and a 91 kDa hypothetical scavenger receptor (AK035662). The DLP and the AP secrete a protein similar to immunoglobulin binding protein (IgBPLP) and one of the experimental autoimmune prostatitis antigen proteins (EAPA2). Peroxiredoxin-6, glucose-regulated protein 78, zinc-a2-glycoprotein and phospholipase Ca are also secreted. Castration of animals led to a decrease in the mRNAs of these secreted proteins, although the extents of change varied greatly among different lobes. We present here an overview of mouse prostate secretion, which should contribute to an understanding of the biological functions of the prostate gland, as well as the androgen-dependency of prostate secretion.
著者キーワード
mouse prostate
secretion
proteome analysis
lobe specific
androgen regulation
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier
発行日 2006-09
権利情報
Copyright (c) 2006 Elsevier Ltd.
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0022-0795
[DOI] 10.1677/joe.1.06733
[NCID] AA00697140
[PMID] 17003280
[DOI] http://dx.doi.org/10.1677/joe.1.06733 ~の異版である