Effects of insulin, triiodothyronine and fat soluble vitamins on adipocyte differentiation and LPL gene expression in the stromal-vascular cells of red sea bream, Pagrus major

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 144 巻 3 号 326-333 頁 2006-07 発行
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タイトル ( eng )
Effects of insulin, triiodothyronine and fat soluble vitamins on adipocyte differentiation and LPL gene expression in the stromal-vascular cells of red sea bream, Pagrus major
作成者
Oku Hiromi
Tokuda Masaharu
Okumura Takuji
収録物名
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
144
3
開始ページ 326
終了ページ 333
抄録
Various kinds of hormones including insulin, triiodothyronine (T3) and fat-soluble vitamins have been proposed as mediators of adipocyte differentiation in mammals. To investigate the factors which are responsible for fish adipocyte differentiation, we developed a serum-free culture system of stromal-vascular cells of red sea bream adipose tissue and examined the effects of insulin (bovine), T3, and fat-soluble vitamins (all-trans retinoic acid, retinyl acetate and 1,25-dihydroxyvitamin D3) on the differentiation-linked expression of the lipoprotein lipase (LPL) gene. As assessed by the increase in LPL gene expression after 3 day cultivation, like in mammalian adipocytes, insulin enhanced the adipocyte differentiation in a concentration-dependent manner. During 2 week cultivation, bovine insulin promoted lipid accumulation in differentiating adipocytes concentration-dependently until the terminal differentiation. These results indicate that the differentiation of fish adipocytes is inducible by insulin alone. T3 alone had no effect but enhanced the differentiation-linked LPL gene expression in the presence of insulin. Fat-soluble vitamins, unlike in mammalian adipocytes, did not show any significant effects. The method developed in this study should be of interest for the characterization of factors involved in fish adipocyte differentiation.
著者キーワード
adipocyte
stromal-vascular cells
differentiation
lipoprotein lipase
LPL
teleost
red sea bream
Pagrus major
NDC分類
水産業 [ 660 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier
発行日 2006-07
権利情報
Copyright (c) 2006 Elsevier Ltd.
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 1096-4959
[DOI] 10.1016/j.cbpb.2006.03.008
[NCID] AA11045017
[PMID] 16716627
[DOI] http://dx.doi.org/10.1016/j.cbpb.2006.03.008 ~の異版である