C-terminal repetitive motifs in Vp130 present at the unique vertex of the Chlorovirus capsid are essential for binding to the host Chlorella cell wall

Virology 353 巻 2 号 433-442 頁 2006-09 発行
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タイトル ( eng )
C-terminal repetitive motifs in Vp130 present at the unique vertex of the Chlorovirus capsid are essential for binding to the host Chlorella cell wall
作成者
Onimatsu Hideki
Suganuma Kei
Uenoyama Shushi
収録物名
Virology
353
2
開始ページ 433
終了ページ 442
抄録
Previously, Vp130, a chloroviral structural protein, was found to have host-cell-wall-binding activity for NC64A-viruses (PBCV-1 and CVK2). In this study, we have isolated and characterized the corresponding protein from chlorovirus CVGW1, one of Pbi-viruses that have a different host range. In NC64A-viruses, Vp130 consists of a highly conserved N-terminal domain, internal repeats of 70-73 aa motifs and a C-terminal domain occupied by 23-26 tandem repeats of a PAPK motif. However, CVGW1 was found to have a slightly different Vp130 construction where the PAPK repeats were not in the C-terminus but internal. Immunofluorescence microscopy with a specific antibody revealed that the C-terminal region containing the Vp130 repetitive motifs from PBCV-1 and CVK2 was responsible for binding to Chlorella cell walls. Furthermore, by immunoelectron microscopy and immunofluorescence microscopy, Vp130 was localized at a unique vertex of the chlorovirus particle and was found to be masked through binding to the host cells. These results suggested that Vp130 is localized at a unique vertex on the virion, with the C-terminal repetitive units outside for cell wall binding.
著者キーワード
C-terminal repetitive motif
Cell wall binding protein
Chlorovirus
Unique vertex
Vp130
NDC分類
植物学 [ 470 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier Inc.
発行日 2006-09
権利情報
Copyright (c) 2006 Elsevier Inc.
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0042-6822
[DOI] 10.1016/j.virol.2006.06.010
[NCID] AA00884454
[DOI] http://dx.doi.org/10.1016/j.virol.2006.06.010 ~の異版である