Scanning X-ray Microscopy of Living and Freeze-Dried Blood Cells in Two Vanadium-Rich Ascidian Species, Phallusia mammillata and Ascidia sydneiensis samea

Zoological science 19 巻 1 号 27-35 頁 2002 発行
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タイトル ( eng )
Scanning X-ray Microscopy of Living and Freeze-Dried Blood Cells in Two Vanadium-Rich Ascidian Species, Phallusia mammillata and Ascidia sydneiensis samea
作成者
Takemoto Kuniko
Fayard Barbara
Salomé Murielle
Yamamoto Akitsugu
Kihara Hiroshi
Susini Jean
Scippa Silvia
Uyama Taro
Michibata Hitoshi
収録物名
Zoological science
19
1
開始ページ 27
終了ページ 35
抄録
Some ascidians (sea squirts) accumulate the transitional metal vanadium in their blood cellsat concentrations of up to 350 mM, about 10 7 times its concentration found in seawater. There are approx-imately 10 different types of blood cell in ascidians. The identity of the true vanadium-containing blood cell(vanadocyte) is controversial and little is known about the subcellular distribution of vanadium. A scanningx-ray microscope installed at the ID21 beamline of the European Synchrotron Radiation Facility to visualizevanadium in ascidian blood cells. Without fixation, freezing or staining realized the visualization of vana-dium localized in living signet ring cells and vacuolated amoebocytes of two vanadium-rich ascidian spe-cies, Phallusia mammillata and Ascidia sydneiensis samea . A combination of transmission andfluorescence images of signet ring cells suggested that in both species the vacuoles contain vanadium.
著者キーワード
vanadium
ascidians
vanadocytes
X-Ray microscopy
synchrotron radiation
NDC分類
動物学 [ 480 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Zoological Society of Japan
発行日 2002
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0289-0003
[NCID] AA10545874