Type-II 3-oxoacyl-CoA thiolase of the nematode Caenorhabditis elegans is located in peroxisomes, highly expressed during larval stages and induced by clofibrate

European Journal of Biochemistry 264 巻 2 号 509-515 頁 1999 発行
アクセス数 : 720
ダウンロード数 : 157

今月のアクセス数 : 2
今月のダウンロード数 : 3
ファイル情報(添付)
ejb_264_509.pdf 5.55 MB 種類 : 全文
タイトル ( eng )
Type-II 3-oxoacyl-CoA thiolase of the nematode Caenorhabditis elegans is located in peroxisomes, highly expressed during larval stages and induced by clofibrate
作成者
Maebuchi Motohiro
Togo Summnanuna H.
Yokota Sadaki
Ghenea Simona
Bun-ya Masanori
Kamiryo Tatsuyuki
Kawahara Akira
収録物名
European Journal of Biochemistry
264
2
開始ページ 509
終了ページ 515
抄録
We examined the expression and localization of type-II 3-oxoacyl-CoA thiolase in the nematode Caenorhabditis elegans. Type-II thiolase acts on 3- oxoacyl-CoA esters with a methyl group at the α carbon, whereas conventional thiolases do not. Mammalian type-II thiolase, which is also termed sterol carrier protein x (SCPx) or SCP2/3-oxoacyl-CoA thiolase, is located in the peroxisomes and involved in phytanic acid degradation and most probably in bile acid synthesis. The nematode enzyme lacks the SCP2 domain, which carries the peroxisomal-targeting signal, but produces bile acids in a cell-free system. Northern and Western blot analyses demonstrated that C. elegans expressed type-II thiolase throughout its life cycle, especially during the larval stages, and that the expression was significantly enhanced by the addition of clofibrate at 5 mM or more to the culture medium. Whole-mount in situ hybridization and immunostaining of L4 larvae revealed that the enzyme was mainly expressed in intestinal cells, which are multifunctional like many of the cell types in C. elegans. Subcellular fractionation and indirect immunoelectron microscopy of the nematode detected the enzyme in the matrix of peroxisomes. These results indicate the fundamental homology between mammalian SCPx and the nematode enzyme regardless of whether the SCP2 part is fused, suggesting their common physiological roles.
著者キーワード
type-II 3-oxoacyl-CoA thiolase
SCPx
peroxisomes
clofibrate
caenorhabditis elegans
NDC分類
生物科学・一般生物学 [ 460 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Federation of European Biochemical Societies
発行日 1999
権利情報
Copyright (c) 1999 Federation of European Biochemical Societies
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0014-2956
[NCID] AA00639541
[DOI] 10.1046/j.1432-1327.1999.00655.x
[PMID] 10491098
[DOI] http://dx.doi.org/10.1046/j.1432-1327.1999.00655.x ~の異版である