Isolation and characterization of coagulant extracted from moringa oleifera seed by salt solution

Water Research 35 巻 2 号 405-410 頁 2001-02 発行
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タイトル ( eng )
Isolation and characterization of coagulant extracted from moringa oleifera seed by salt solution
作成者
Baes Aloysius U.
Okada Mitsumasa
収録物名
Water Research
35
2
開始ページ 405
終了ページ 410
著者キーワード
coagulation
purification
isolation
characterization
moringa olelfera
内容記述
It is known that M. oleifera contains a natural coagulant in the seeds. In our previous research, the method using salt water to extract the active coagulation component from M. oleifera seeds was developed and compared with the conventional method using water. In this research, the active coagulation component was purified from a NaCl solution crude extract of Moringa oleifera seeds. The active component was isolated and purified from the crude extract through a sequence of steps that included salting-out by dialysis, removal of lipids and carbohydrates by homogenization with acetone, and anion exchange. Specific coagulation activity of the active material increased up to 34 times more than the crude extract after the ion exchange. The active component was not the same as that of water extract. The molecular weight was about 3000 Da. The Lowry method and the phenol-sulfuric acid method indicated that the active component was neither protein nor polysaccharide. The optimum pH of the purified active component for coagulation of turbidity was pH 8 and above. Different from the conventional water extracts, the active component can be used for waters with low turbidity without increase in the dissolved organic carbon concentration.
NDC分類
公害・環境工学 [ 519 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier
発行日 2001-02
権利情報
Copyright (c) 2000 Elsevier Science Ltd.
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0043-1354
[DOI] http://dx.doi.org/10.1016/S0043-1354(00)00290-6 ~の異版である