VanabinP, a novel vanadium-binding protein in the blood plasma of an ascidian, Ascidia sydneiensis same

Biochimica et Biophysica Acta 1730 巻 206-217 頁 2005 発行
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タイトル ( eng )
VanabinP, a novel vanadium-binding protein in the blood plasma of an ascidian, Ascidia sydneiensis same
作成者
Yoshihara Masao
Watanabe Takahiro
Yamaguchi Nobuo
Kamino Kei
Michibata Hitoshi
収録物名
Biochimica et Biophysica Acta
1730
開始ページ 206
終了ページ 217
抄録
Some ascidians accumulate high levels of the transition metal vanadium in their blood cells. The process of vanadium accumulation has not yet been elucidated. In this report, we describe the isolation and cDNA cloning of a novel vanadium-binding protein, designated as VanabinP, from the blood plasma of the vanadium-rich ascidian, Ascidia sydneiensis samea. The predicted amino acid sequence of VanabinP was highly conserved and similar to those of other Vanabins. The N-terminus of the mature form of VanabinP was rich in basic amino acid residues. VanabinP cDNA was originally isolated from blood cells, as were the other four Vanabins. However, Western blot analysis revealed that the VanabinP protein was localized to the blood plasma and was not detectable in blood cells. RT-PCR analysis and in situ hybridization indicated that the VanabinP gene was transcribed in some cell types localized to peripheral connective tissues of the alimentary canal, muscle, blood cells, and a portion of the branchial sac. Recombinant VanabinP bound a maximum of 13 vanadium(IV) ions per molecule with a Kd of 2.8 × 10-5 M. These results suggest that VanabinP is produced in several types of cell, including blood cells, and is immediately secreted into the blood plasma where it functions as a vanadium(IV) carrier.
著者キーワード
Ascidian
Vanadium
Metal accumulation
Blood plasma
NDC分類
生物科学・一般生物学 [ 460 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier B.V.
発行日 2005
権利情報
Copyright (c) 2005 Elsevier B.V.
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0167-4781
[NCID] AA10506089
[PMID] 16084607
[DOI] 10.1016/j.bbaexp.2005.07.002
[DOI] http://dx.doi.org/10.1016/j.bbaexp.2005.07.002 ~の異版である