The purpose of present study was to examine perceptions towards inclusion of students with disabilities among inservice teachers working in mainstream primary schools of southern Bangladesh. This paper reviewed the prevailing situation of education of students with disabilities in mainstream education setting. A number of 62 in-service teachers of 18 mainstream schools in four southern districts participated in this study. This article was based on selfrated questionnaires that were distributed to participant teachers. Also, focus group discussion was organized in each school. The data were analyzed using descriptive statistics such as frequency and percentages. The major findings of the study revealed that in-service teachers had positive attitudes towards inclusion of students with disabilities in mainstream classroom. The results of this study also have significant implications on how to develop in-service teachers' professional capacities for quality teaching-learning in inclusive classroom in Bangladesh.