Deficiency of nitrogen, phosphoric acid and potassium results in a substantial decrease in the concentration of ascorbic acid. Especially potassium and nitrogen reduce, but phosphoric acid has no obvious effect. Fertilizing together with lime increases the ascorbic acid content in leaves of Russian comfrey. Single trace element in the case of Cu increases the ascorbic acid content of Russian comfrey as in many other kinds of plants. The ascorbic acid content of Russian comfrey is uniform under the same conditions in different soils.