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広島大学水畜産学部紀要 Volume 11 Issue 1
published_at 1972-07-31
海底泥の硫酸還元活性に関する一実験
An Experiment on the Sulfate Reducing Activity of Sea Bottom Mud
Asakawa Suezo
Abstract
古坂が水田土壌中の硫酸還元菌群の活性度をワールブルグ検圧計を用いて測定する方法を考案した.それを養殖場の海泥に応用した.即ち,
1. 海泥中に常在する呼吸基質を洗瀞除去する前処理を加えるならば,古坂の法が応用できることを知った.
2. 養殖場内外の海泥につき夏,秋および冬季について測定した結果,硫酸還元菌群の活性は2cmぐらいまでの表層部には全く認められず,10cmほどの深層部では夏季よりも秋季に強い傾向があり,冬季は全く認められなかった.
3. 硫酸還元菌群の活性の強弱と硫化物量とは必ずしも相関しないことを論じた.
1. 海泥中に常在する呼吸基質を洗瀞除去する前処理を加えるならば,古坂の法が応用できることを知った.
2. 養殖場内外の海泥につき夏,秋および冬季について測定した結果,硫酸還元菌群の活性は2cmぐらいまでの表層部には全く認められず,10cmほどの深層部では夏季よりも秋季に強い傾向があり,冬季は全く認められなかった.
3. 硫酸還元菌群の活性の強弱と硫化物量とは必ずしも相関しないことを論じた.
Abstract
A method of the Warburg's manometry was reported by C. FURUSAKA for the evaluation of the activity of a sulfate reducer in paddy field soil. It was found that this method could be applied for sea bottom mud, if the mud were washed thrice in advance with a non-oxygen 3% sodium chloride solution in order to remove the sulfate present in the sample.
According to his definition, the activity of hydrogen uptake was designated as a hydrogenase activity of mud (HA value in Table 1). In addition of a sulfite solution to the mud a significant increase in the rate of hydrogen uptake was further observed, the increment caused by the addition of the sulfite solution was designated as a sulfate reducing activity of mud (SRA value in Table 2), which is caused by the action of sulfate reducing bacteria in the mud.
The mud samples collected from a fish farm bottom in the Seto Inland Sea were determined by a modified method. Considering the results in Table 2, there was no activity (SRA) at the layer from 0 to 2 cm mud depth, but considerable activity at the 10 cm mud depth. Moreover, it was suggested that the activities varied seasonally, rather higher in autumn than in summer and null in winter.
According to his definition, the activity of hydrogen uptake was designated as a hydrogenase activity of mud (HA value in Table 1). In addition of a sulfite solution to the mud a significant increase in the rate of hydrogen uptake was further observed, the increment caused by the addition of the sulfite solution was designated as a sulfate reducing activity of mud (SRA value in Table 2), which is caused by the action of sulfate reducing bacteria in the mud.
The mud samples collected from a fish farm bottom in the Seto Inland Sea were determined by a modified method. Considering the results in Table 2, there was no activity (SRA) at the layer from 0 to 2 cm mud depth, but considerable activity at the 10 cm mud depth. Moreover, it was suggested that the activities varied seasonally, rather higher in autumn than in summer and null in winter.
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