Information on the nematodes of freshwater fishes of Japan published between 1905 and 2016 is assembled as Parasite-Host and Host-Parasite lists. Fifty-two nominal species and some unidentified species of nematodes in 17 families, three orders, and two classes were reported. This is the second part of the checklist, which includes 22 nominal species in the following seven families in the order Spirurida (class Chromadorea): Hedruridae (1 species), Micropleuridae (1 species), Philometridae (7 species), Physalopteridae (1 species), Quimperiidae (2 species), Raphidascarididae (4 species), and Rhabdochonidae (6 species). Information on the nematodes not identified to family level is also included.