We investigated the butterfly fauna in the Higashi-Hiroshima campus of Hiroshima University using route census surveys conducted from April to November 2015. Fifty-six species belonging to five families were recorded in this study. Ten additional species were found outside the survey period, which resulted in the detection of 66 species of butterflies (five families). The species richness was the highest in the farm field adjacent to the natural forest; however, the detected butterfly species differed from one area to another. Considering previous surveys made in this campus, the present results suggested slight environmental changes in and around the Higashi-Hiroshima campus.