We conducted a yearlong route census to survey amphibian fauna in the Higashi-Hiroshima campus of Hiroshima University from April 2015 to April 2016. A total of 2 orders, 7 families, and 10 species were recorded in this survey; 5 species (Hynobius nebulosus, Cynopus pyrrhogaster, Bufo japonicus japonicus, Rana japonica, Pelophylax nigromaculatus) were listed in the Red Data Book of Hiroshima Prefecture. Although species compositions were distinct for each area, alien species such as Lithobates catesbeianus or Procambarus clarkii were observed in many areas, so there is concern that these alien species may have a negative impact on the native ecosystem.