Specimens of Lernaea cyprinacea Linnaeus, 1758 were collected from the anal fin of dark chub (Candidia temminckii) and from the gill membrane and caudal peduncle of bluegill (Lepomis macrochirus) collected from the Masuda River, a tributary of the Sasa River within the upper Matsuda River system, in Ehime Prefecture, Shikoku, one of the four major islands of Japan. This collection represents the first and second records for L. cyprinacea in Ehime Prefecture and Shikoku, respectively.