Gas Liquid Chromatography による魚肉腐敗生産物中のアミン類の分離

Separation of lower aliphatic amines present in the spoilt fish meat by gas liquid chromatography

Sato Yoshikazu

Ito Keiji

Matsumoto Fumio

広島大学水畜産学部紀要

Journal of the Faculty of Fisheries and Animal Husbandry, Hiroshima University

(1) アンモニアおよび低級脂肪族アミン類のG. L. C. による分離において， 固定相液体n-Hexadecane， Paraffin wax， Triethylen glycol, Cetylalcohol, Glycerol, Triethanolamine の6種類のうちでは， Cetylalcohol， Glycerol， Triethanolamine が比較的分離能のすぐれていることを知った.
(2) 固定相液体としてTriethanolamine 1m， Cetylalcohol 4m を直列につないだものを使用してアンモニアと10 種類の低級脂肪族アミンを分離し，その場合のカラム温度，キャリヤーガス流速の適度はそれぞれ98 ℃，30ml/min. と定めた.
(3) 上記のカラムを用いてフイッシュソルプルの腐敗生産物から，アンモニア， トリメチルアミン，メチルアミン，エチノレアミン，ジエチルアミン， トリエチルアミン又はイソプチルアミンの6種類を確認し得た.量的な関係はアンモニアが大部分で次I"Cトリメチルアミンが多く，微量のメチルアミンおよびヱチルアミン， トリエチルアミン又はイソプチルアミンの痕跡が認められた.

In this study, the authors attempted to separate the lower aliphatic amines and ammonia present in the spoilt fish meat by using of Gas-Liquid Chromatography (G. L. C.). However, the separation is difficult and unsuccessful unless the adopted partition liquid is suitable. So the liquid phase for available has been investigated, and determined the column temperature and the carrier gas flow rate. Then the separation of a standard mixture of aliphatic primary, secondary and tertiary amines were achieved reasonably, and the detection of amines content in a spoilt fishsoluble were
taken for a test of the method established above.

The results are as follows:

(1) As partition liquid for the separation of lower aliphatic amines, triethanolamine, cetylalcohol and glycerol were excellent than n-hexadecane, paraffin wax and triethylen glycol. (Text-fig. 1.)
(2) Especially the column connected with 1 m triethanolamine and 4 m cetylalcohol was more effective. In this case, the more suitable condition of procedure was the temperature of column 98°C and the flow rate of carrier gas 30 ml/min. (Text-fig. 2 & 3.)
(3) Although the separation of a standard mixture of aliphatic primary， secondary， tertiary amines and ammonia were achieved reasonably, it is imperfect to estimate them quantitatively because of the tailing of methylamine， dimethylamine and n-buthylamine. (Text-fig. 4.)
(4) By the detection of amines present in a spoilt fish-soluble were obtained remarkable ammonia, considerable trimethylamine and a little methylamine, ethylamine, diethylamine and trimethylamine or iso-buthylamine. (Text-fig.5-1~3.)

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jpn

広島大学水畜産学部

[ISSN] 0440-8756

[NCID] AN00213563