瀬戸内海産無腸動物 Praesagittifera naikaiensis の飼育システム <論文>

Artificial Rearing System for Praesagittifera naikaiensis (Acoela, Acoelomorpha) <Article>

彦坂(片山) 智恵

彦坂 暁

広島大学大学院総合科学研究科紀要. I, 人間科学研究

Bulletin of the Graduate School of Integrated Arts and Sciences, Hiroshima University. I, Studies in human sciences

Praesagittifera naikaiensis is an acoelomorph worm native to the seashore of the Seto Inland Sea. P. naikaiensis harbors a species of prasinophytes, Tetraselmis sp., as a symbiont. The symbiotic algae are acquired horizontally from the environment during the larval stage. In this study, we established a method for the rearing of adult P. naikaiensis as a first step toward using the animal in the study of development, evolution, and the symbiotic mechanism. We kept the animals in aquaria with closed seawater circulations. The bottom of the aquarium was half covered with sand collected from the seashore of the Seto Inland Sea. An LED light was suspended over the aquarium to aid photosynthesis. The system was maintained at a salinity of 32–33 ppt and a temperature of 14°C, on a 14-h light (35–80 μmol quanta m−2s−1)/10-h dark photoperiod. We succeeded in constantly producing sexually mature animals, independent of season. The system developed here will serve as a foundation for future studies on acoel evolution and development.

Acoelomorpha

Praesagittifera naikaiensis

Sex maturation

Development

Symbiosis

Evo–Devo

動物学 [ 480 ]

日本語

広島大学大学院総合科学研究科

Copyright (c) 2015 by Graduate School of Integrated Arts and Sciences, Hiroshima University All rights reserved

[ISSN] 1881-7688

[NCID] AA12198647