Detection of organophosphorus compound based on a sol-gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase

Applied Physics Letters Volume 98 Issue 23 Page 233503-1-233503-3 published_at 2011-06-07
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Title ( eng )
Detection of organophosphorus compound based on a sol-gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase
Creator
Enami Yasufumi
Tsuchiya Keiji
Suye Shin-ichiro
Source Title
Applied Physics Letters
Volume 98
Issue 23
Start Page 233503-1
End Page 233503-3
Abstract
In this letter, the authors report the real-time detection of an organophosphorus compound using a sol-gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase on a yeast-cell surface display. The waveguide was pumped at 488 nm, and it emitted green fluorescence at the far field. The green fluorescent light at 550 nm changed by 50% from the original power 1 min after application of the organophosphorus compound. The results enable the real-time detection of sarin and other biochemicals by using an in-line fiber sensor network.
Language
eng
Resource Type journal article
Publisher
American Institute of Physics
Date of Issued 2011-06-07
Rights
Copyright (c) 2011 American Institute of Physics.
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0003-6951
[DOI] 10.1063/1.3596448
[NCID] AA00543431
[DOI] http://dx.doi.org/10.1063/1.3596448