Recombinant Protein Synthesis for Nanomaterial Assembly: Technical Overview

ナノ学会会報 Volume 20 Issue 2 Page 31-37 published_at 2022-03
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Title ( eng )
Recombinant Protein Synthesis for Nanomaterial Assembly: Technical Overview
Creator
Nakano Ayana
Source Title
ナノ学会会報
The bulletin of the Society of Nano Science and Technology
Volume 20
Issue 2
Start Page 31
End Page 37
Abstract
Recombinant DNA technology has emerged as a useful method for providing protein-based building blocks for nanomaterial assembly. This article provides an overview of the technical aspects of recombinant protein synthesis, especially for nanomaterial scientists and engineers. First, the rationale for the use of protein-based nanomaterials synthesized through recombinant DNA technology is presented. This article then focuses on simple standard protocols for protein expression in Escherichia coli. The last section presents several methods for creating artificial proteins with complex structures.
Descriptions
This work was supported by Grant-in-Aid for Research Activity Start-up (No. 2l K2l0l4) and Fund for the Promotion of Joint Intemational Research (No. 19KK0278), JSPS.
Language
eng
Resource Type journal article
Publisher
ナノ学会
Date of Issued 2022-03
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 1347-8028