Decreased mitochondrial translation confers 3,3′-Diindolylmethane resistance to Schizosaccharomyces pombe

Wang Kaiyu

Nagai Hideto

Rajib Samiul Alam

Satou Yorifumi

Ueno Masaru

Biochemical and Biophysical Research Communications

3,3′-Diindolylmethane (DIM), a compound derived from natural fruits and vegetables, is widely recognized for its anti-cancer activity. However, its action mechanisms remain ambiguous. In this study, to study the molecular mechanism of 3,3′-Diindolylmethane, we identified a novel mutation in the gene of mitochondrial translation elongation factor EF-Ts (tsf1+), a key factor in mitochondrial protein translation, that conferred DIM resistance to Schizosaccharomyces pombe. The tsf1Δ also conferred DIM resistance. Decreased mitochondrial translation was found to be responsible for conferring DIM resistance to Schizosaccharomyces pombe, as the cells gained DIM resistance after treatment with chloramphenicol, a specific mitochondrial translation inhibitor. Notably, tsf1Δ conferred DIM resistance in the absence of either autophagy-related protein, Atg7, or nuclear envelope protein, Lem2, two proteins that have been reported to be required for cell survival in the presence of DIM. Overall, this study revealed novel biological functions of DIM and highlighted its potential as an anti-cancer agent.

Mitochondria

Tsf1

Translation elongation factor

3,3′-Diindolylmethane

eng

Elsevier

© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/

This is not the published version. Please cite only the published version.

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[DOI] https://doi.org/10.1016/j.bbrc.2024.150864 isVersionOf

日本学術振興会

Japan Society for the Promotion of Science

[Crossref Funder] https://doi.org/10.13039/501100001691

がん転移阻止を目指したブロッコリー由来成分DIMによる核膜損傷機構の解明

がん転移阻止を目指したブロッコリー由来成分DIMによる核膜損傷機構の解明

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