A Simple Method for the Observation of Cultured Rat Hepatocytes by Scanning Electron Microscopy
Hiroshima Journal of Medical Sciences Volume 36 Issue 2
Page 169-174
published_at 1987-06
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| File | |
| Title ( eng ) |
A Simple Method for the Observation of Cultured Rat Hepatocytes by Scanning Electron Microscopy
|
| Creator |
KASUGA Shigeo
TAGASHIRA Nobuharu
HARADA Yasuo
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 36 |
| Issue | 2 |
| Start Page | 169 |
| End Page | 174 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
A simple method for the three-dimensional observation of inner and outer ultrastructures of the cultured hepatocyte was presented. The freeze cracked plane of the cultured cell was obtained by means of cracking the underlying substrate. This method made it possible to observe the ultrastructural features of both cell surface and cytoplasm in the same cell. Among several substrates examined in this investigation, the collagen gel sheet was the most useful for this purpose.
|
| Keywords |
Cultured hepatocytes
Scanning electron microscopy
Three-dimensional structure
Aldehyde-prefix osmium-DMSO-osmium method
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima Journal Medical Press
|
| Date of Issued | 1987-06 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 3654268
|
