Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres
PLoS ONE Volume 10 Issue 10
Page e0140456-
published_at 2015-10-14
アクセス数 : 496 件
ダウンロード数 : 69 件
今月のアクセス数 : 6 件
今月のダウンロード数 : 3 件
この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00048778
| File | |
| Title ( eng ) |
Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres
|
| Creator |
Nanbu Tomoko
Nguyễn Luân C.
Habib Ahmed G. K.
Hirata Naoya
Ukimori Shinobu
Tanaka Daiki
Masuda Kenta
Takahashi Katsunori
|
| Source Title |
PLoS ONE
|
| Volume | 10 |
| Issue | 10 |
| Start Page | e0140456 |
| Abstract |
The uncapping of telomeres induces a DNA damage response. In Schizosaccharomyces pombe, deletion of pot1+ causes telomere uncapping and rapid telomere resection, resulting in chromosome fusion. Using the nmt-pot1-aid strain, we previously reported that Pot1 shut-off causes telomere loss and chromosome fusion in S. pombe. However, the factors responsible for the resection of uncapped telomeres remain unknown. In this study, we investigated these factors and found that concomitant deletion of rqh1+ and exo1+ alleviated the loss of telomeres following Pot1 shut-off, suggesting that Rqh1 and Exo1 are redundantly involved in the resection of uncapped telomeres. We also investigated the role of Rqh1 helicase activity and found it to be essential for the resection of uncapped telomeres. Moreover, we found that Dna2 and Exo1 function redundantly in the resection of uncapped telomeres. Taken together, these results suggest that Exo1 and Rqh1-Dna2 redundantly contribute to the resection of uncapped telomeres. Therefore, our results demonstrate that nmt-pot1-aid is an important model strain to study the role of helicases and nucleases in the resection of uncapped telomeres and to improve our understanding of DNA double-strand break repair.
|
| Descriptions |
This research is (partially) supported by the Platform Project for Supporting Drug Discovery and Life Science Research (Platform for Dynamic Approaches to Living System) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), and Japan Agency for Medical Research and Development (AMED), and by Grants-in-Aid for Scientific Research in Priority Areas, provided to M. U. from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
|
| Language |
eng
|
| Resource Type | journal article |
| Publisher |
Public Library of Science
|
| Date of Issued | 2015-10-14 |
| Rights |
© 2015 Nanbu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
|
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 1932-6203
[DOI] 10.1371/journal.pone.0140456
[DOI] https://doi.org/10.1371/journal.pone.0140456
|
