An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation

Journal of Cell Science Volume 129 Issue 24 Page 4592-4606 published_at 2016

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Title ( eng )	An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation
Creator	Matsuo Yuzy Maurer Sebastian P. Yukawa Masashi Zakian Silva Singleton Martin R. Surrey Thomas Toda Takashi
Source Title	Journal of Cell Science
Volume	129
Issue	24
Start Page	4592
End Page	4606
Abstract	Dynamic microtubule plus-ends interact with various intracellular target regions such as the cell cortex and the kinetochore. Two conserved families of microtubule plus-end-tracking proteins, the XMAP215, ch-TOG or CKAP5 family and the end-binding 1 (EB1, also known as MAPRE1) family, play pivotal roles in regulating microtubule dynamics. Here, we study the functional interplay between fission yeast Dis1, a member of the XMAP215/TOG family, and Mal3, an EB1 protein. Using an in vitro microscopy assay, we find that purified Dis1 autonomously tracks growing microtubule ends and is a bona fide microtubule polymerase. Mal3 recruits additional Dis1 to microtubule ends, explaining the synergistic enhancement of microtubule dynamicity by these proteins. A non-canonical binding motif in Dis1 mediates the interaction with Mal3. X-ray crystallography shows that this new motif interacts in an unconventional configuration with the conserved hydrophobic cavity formed within the Mal3 C-terminal region that typically interacts with the canonical SXIP motif. Selectively perturbing the Mal3–Dis1 interaction in living cells demonstrates that it is important for accurate chromosome segregation. Whereas, in some metazoans, the interaction between EB1 and the XMAP215/TOG family members requires an additional binding partner, fission yeast relies on a direct interaction, indicating evolutionary plasticity of this critical interaction module.
Keywords	Crystallography EB1 Microtubule polymerase TIRF microscopy XMAP215 TOG
Descriptions	This work was supported by Cancer Research UK and the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001155, FC001163, FC001184), the UK Medical Research Council (FC001155, FC001163, FC001184), and the Wellcome Trust (FC001155, FC001163, FC001184), the Japan Society for the Promotion of Science (JSPS) [KAKENHI Scientific Research (A) (16H02503 to T.T.), a Challenging Exploratory Research grant (16K14672 to T.T.), Scientific Research (C) (16K07694 to M.Y.)], the Naito Foundation (T.T.) and a Marie Curie fellowship from the European Commission (PIEF-GA-2009-253043 to S.P.M.). Supplementary information available online at http://jcs.biologists.org/lookup/doi/10.1242/jcs.197533.supplemental
Language	eng
Resource Type	journal article
Date of Issued	2016
Rights	© 2016. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Publish Type	Version of Record
Access Rights	open access
Source Identifier	[ISSN] 0021-9533 [ISSN] 1477-9137 [DOI] 10.1242/jcs.197533 [DOI] https://doi.org/10.1242/jcs.197533 [PMID] 27872152